📋 Tender Overview
Plasmodesmata, Symplasmic Pores For Plant Cell-To-Cell Communication
Novel Technologies To Study The Unique Plant Cell–Cell Communicationmulticellular Organisms Have Channels For Nutrient Exchange And Communication Between Cells. Plants Developed Plasmodesmata, Unique Complex Cell–Cell Connections Traversing The Cell Walls. Plasmodesmata Are Involved In The Selective Transport Of Signals, Ions, Metabolites, Rnas And Proteins. Presently, The Composition, Structure And Regulation Of Plasmodesmatal Conductance Remain Poorly Understood. Novel Technologies Are Now Setting The Stage For Resolving The Roles Of Plasmodesmata In Transport And Signalling Using An Interdisciplinary Approach. The Eu-Funded Sympore Project Will Use Novel Technologies Of Proximity Labelling Proteomics To Obtain Plasmodesmatal Composition And Electron Tomography For Structure Elucidation. As Plasmodesmata Play Key Roles In Plant Nutrient Allocation And Virus Spread, This Project Will Enable Novel Biotech Solutions In Agriculture.
Funded Under
Excellent Science - European Research Council (Erc)
Total Cost
€ 10 582 000,00
Eu Contribution
€ 10 582 000,00
Coordinated By
Heinrich-Heine-Universitaet Duesseldorf
Germany
Start Date
1 April 2021
End Date
31 March 2027
Objective
During evolution of multicellularity, cells differentiated to become specialized and interdependent. Multicellular organisms invented channels for nutrient exchange and communication between cells. Plants uniquely developed plasmodesmata, complex cell-cell connections traversing the cell wall. Roles ascribed to plasmodesmata include selective transport of signals, ions, metabolites, RNAs and proteins. Due to technical hurdles, composition, structure and regulation of plasmodesmatal conductance remain enigmatic. Genetic approaches to study plasmodesmata were hampered by lethality or redundancy. Novel technologies now set the stage for resolving roles of plasmodesmata in transport and signaling in an interdisciplinary approach. We will use proximity labeling proteomics to obtain plasmodesmatal composition, and PAINT and cryo electron tomography (cryoET) for near atomic structures. Models of plasmodesmata will be built from bottom up and top down approaches and combined with quantitative assessment of plasmodesmatal activity. Novel biosensor approaches together with knock down by genome editing will permit quantitation of transport of the diverse cargo. Single cell sequencing helps fine-tuning mutant selection and targeting of subtypes. Four labs will join forces: highly recognized experts in biophysics and cryoET (WB), advanced imaging and developmental signaling (RS), high-end proteomics and lipidomics (WS), and interactomics, transporters and cutting-edge biosensor technology (WF). We will iteratively address: (1) systematic quantitative identification of components, (2) their localization and dynamics, (3) structures and molecular building blocks of diverse plasmodesmatal types, and (4) trans
