📋 Tender Overview
Mechanisims Of Nuclear Self-Assembly
Objective
Shape and function of the vertebrate nucleus depend on the choreographed interplay between lipids, proteins, and DNA to form the nuclear envelope. Even small molecular changes, such as point mutations in the proteins of the nuclear envelope (the lamina) cause detrimental human diseases including premature aging, cancer, and heart disease. To date, a clear, mechanistically compelling explanation for the dynamic coupling of lipids, proteins, and DNA to safeguard nuclear shape and function is still missing. We here propose to build minimal, synthetic nuclei (‘Organelloids’) bottom-up as a tool to study the self-assembly of a functional nucleus.
We recently discovered the conserved molecular machinery that ensures the assembly of the nuclear envelope in open vertebrate mitosis. Our data point to uncharacterized fundamental mechanisms that couple the fusion of lipid-membrane sheets into a continues nuclear membrane to the formation of the lamina, and the organization of chromatin in the same chain of events.
Funded Under
European Research Council (Erc)
Total Cost
€ 1 499 974,00
Eu Contribution
€ 1 499 974,00
Coordinated By
Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften Ev
Germany
