Japan Murata Ionissimo Tender

Murata Manufacturing Co. Ltd. hereinafter Murata commissioned Nara Medical University to research the effectiveness of ozone gas generated by Muratas Ionissimo ionizer module1 and ozonizer module2 hereinafter the products in inactivating the novel coronavirus SARS-CoV-23. The ozone gas generated by the products was proven to be effective in inactivating the coronavirus SARS-CoV-2. This confirmation of the Ionizer Modules effectiveness was conducted using an airtight testing chamber its effectiveness in an actual usage environment outside the testing chamber has not yet been tested. Tested productsIonizer modules MHM306 series and MHM402 seriesOzonizer modules MHM500 seriesTesting contractorNara Medical University Department of Microbiology and Infectious Diseases School of Medicine Professor Hisakazu Yano and Professor Ryuichi Nakano Confirmation of effectiveness of the Ionissimo Ionizer Module in inactivating the novel coronavirusData Testing period August to September 2020 Subject Novel coronavirus SARS-CoV-2Methodology 20 l of fluid containing the virus was applied to a petri dish and left to stand until dry. The test piece containing the virus was left to stand in a 6.7-liter airtight chamber and exposed for a fixed period of time to ozone generated by the ozonizer and propelled by a fan. Only a single test piece was placed in the chamber and removed when the reaction time had elapsed. After the test piece was removed the procedure was reset and a new test piece placed in the chamber with the reaction time starting again from 0 minutes. After the reaction time had elapsed 2 ml of SCDLP culture medium4 was dripped onto the test piece and a cell scraper was used to collect the virus. The collected fluid was used to infect Vero E6TMPRSS25 cells and the virus infectivity6 PFUmL was measured using a plaque assay7. Test pieces not exposed to ozone were used as a control. The test was carried out three times in the following environment humidity 50.0 to 59.7 and temperature 19.6C to 21.8C. The ozone concentration value was measured beforehand with no test piece present.ResultsIt was confirmed that at least 99.9 of the virus became inactive following exposure for 120 minutes to a concentration of 0.1 ppm which is the acceptable ozone concentration designated by the Japan Society for Occupational Health.Note The present confirmation of effectiveness was conducted using the products in a testing chamber environment and does not indicate effectiveness in an actual installationusage environment.