📋 Tender Overview
Contract awarded for Delmic meteor 2.0 with super-z lot-0001, title: delmic meteor 2.0 with super-z, description: the cryoem technology platform at the university of hamburg aims to investigate highly complex, thick, and biologically diverse samples – including organoids, c. elegans, plant tissues, and densely growing cell assemblies – with the highest precision using cryo-fib/sem and cryo-electron tomography (cryo-et) at the nanoscale. crucial for the success of these research projects is the reliable localization of the site of interest (soi) under cryogenic conditions directly in the fib/sem. a central methodological bottleneck is the limited accuracy of the existing integrated thermofisher aquilos 2 fluorescence and light microscope (iflm). these limitations particularly affect: ? very small fluorescent target structures (e.g., viruses, membrane contacts, synaptic structures) ? weakly fluorescent structures that are easily overshadowed by background signals ? thick and complex samples, where the z-position of the target structure can only be roughly estimated during the milling process and is often lost ? rare events, such as lipid droplet–endosome contacts or pathogen-host interactions, for which even small coordinate deviations of a few hundred nanometers can determine success or failure. to enable this research project, further development of optical localization is required. this means that the target region must be reliably maintained in the final lamella throughout the entire milling process to subsequently allow for high-resolution imaging in cryo-tem. estimated value excluding vat: 289987.00 eur.Delmic meteor 2.0 with super-z
